Chiral amines kit

The enzymes in this kit (EZK004) have been selected for their activity on a wide selection of substrates and in a wide range of pH, to encounter the different synthetic needs of our customers. Our chiral amines kit contains a comprehensive section of enzymes classes capable of transforming a variety of substrates into primary, secondary and tertiary amines. 

Aromatic and aliphatic primary amines can be obtained using our transaminases. This kit contains 17 transaminase (TA) enzymes for the formal reductive amination of ketone to the corresponding (R)- or (S)-amine. 

Aromatic and aliphatic primary amines can be obtained using amine dehydrogenases (AmDHs). This kit contains 4 amine dehydrogenase enzymes for the reductive amination of ketone with ammonia to the corresponding (R)-amine.

Enantiopure primary, secondary and tertiary amines can be obtained using imine reductase (IREDs). This kit contains 9 imine reductase enzymes for the reduction of imine to amine and the reductive amination of carbonyl to a primary and secondary amine.

Enzyme class  Enzyme Cofactor regeneration Optimum pH
Transaminase STA-1 PLP 8.0 - 9.0
Transaminase STA-2 PLP 6.5 - 7.5
Transaminase STA-13 PLP 6.0 - 8.5
Transaminase STA-14 PLP 6.5 - 10.0
Transaminase STA-113 PLP 8.0 - 8.5
Transaminase STA-118 PLP 8.5 - 9.0
Transaminase STA-120 PLP 8.0 - 8.5
Transaminase STA-121 PLP 8.0 - 8.5
Transaminase RTA-25 PLP 6.5 - 10.0
Transaminase RTA-40 PLP 7.0 - 8.0
Transaminase RTA-45 PLP 6.5 - 7.5
Transaminase RTA-57 PLP 6.0 - 7.0 
Transaminase RTA-58 PLP 7.5 - 8.0
Transaminase RTA-102 PLP  7.0 - 7.5
Transaminase RTA-103 PLP  7.5 - 8.0
Transaminase RTA-104 PLP 7.5 - 8.5
Transaminase RTA-105 PLP 7.0 - 8.0
Amine dehydrogenase AmDH-1  GDH  9.0 -10.0 
Amine dehydrogenase AmDH-2   GDH 9.0 -10.0  
Amine dehydrogenase AmDH-3   GDH  9.0 -10.0  
Amine dehydrogenase AmDH-8   GDH  9.0 -10.0  
Imine reductase  IRED-1 GDH  7.0 - 8.5
Imine reductase   IRED-3 GDH  7.5 - 8.5 
Imine reductase   IRED-17 GDH  7.0 - 8.5
Imine reductase   IRED-18  GDH  7.0 - 8.0
Imine reductase   IRED-33 GDH  7.0 - 8.5
Imine reductase   IRED-44 GDH  7.0 - 8.5
Imine reductase   IRED-49 GDH  8.0 - 8.5
Imine reductase   IRED-69 GDH  6.0 - 7.5
Imine reductase   IRED-72 GDH  6.0 - 7.5
Glucose dehydrogenase GDH-101   - 6.0 - 9.0
Lactate dehydrogenase LDH-4    - 6.0 - 9.0 
Lactate dehydrogenase LDH-12    - 6.0 - 8.0
Alanine dehydrogenase  AlaDH-6   - 6.0 - 9.0
 

We supply each kit with 200mg of each enzyme, except GDH-101, which is 500mg and also comes with a supply of relevant cofactors.

Full enzyme descriptions, reaction setup and work-up instructions, as well as our expert tips, are included with each kit. Please contact us with any questions regarding this kit or its contents and we'll be happy to help.

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