C=O reduction enzyme kit | Johnson Matthey
C=O reduction kit
The enzymes in this kit (EZK003) have been selected for their activity on a wide selection of substrates and in a wide range of pH, to encounter the different synthetic needs of our customers.
Enantiopure alcohols can be obtained using our ADHs. (R) and (S)-enantiomers of acetophenone and its derivatives can be accessed by using these ADHs as well as racemic mixtures. Also, some of our enzymes have been specifically selected for their ability to reduce ketones in bulky substrates, such as benzophenone derivatives.
This kit contains 17 alcohol dehydrogenase (ADH) enzymes for the reduction of ketones and aldehydes to the corresponding alcohols. The enzymes in this kit belong to different protein folds (i.e. short-chain ADHs, zinc-containing ADHs, aldo-ketoreductases), both from prokaryotes and eukaryotes. Also included are enzymes identified from genomic and metagenomic samples and engineered enzymes.
| Enzyme class | Enzyme | Cofactor regeneration | Optimum pH |
| Alcohol dehydrogenase | ADH-19 | GDH, FDH, isopropanol | 6.0 - 7.5 |
| Alcohol dehydrogenase | ADH-20 | GDH, FDH, isopropanol | 6.0 - 6.5 |
| Alcohol dehydrogenase | ADH-27 | GDH, FDH | 6.0 - 9.0 |
| Alcohol dehydrogenase | ADH-61 | GDH, FDH, isopropanol | 6.0 - 9.0 |
| Alcohol dehydrogenase | ADH-62 | GDH | 6.0 - 9.0 |
| Alcohol dehydrogenase | ADH-101 | GDH, isopropanol | 6.5 - 7.5 |
| Alcohol dehydrogenase | ADH-104 | GDH, FDH | 6.0 - 6.5 |
| Alcohol dehydrogenase | ADH-105 | GDH, FDH, isopropanol | 6.0 - 7.0 |
| Alcohol dehydrogenase | ADH-110 | GDH, isopropanol | 6.5 - 7.5 |
| Alcohol dehydrogenase | ADH-150 | GDH, FDH, isopropanol | 6.5 - 9.5 |
| Alcohol dehydrogenase | ADH-153 | GDH | 6.0 - 9.5 |
| Alcohol dehydrogenase | ADH-159 | GDH, FDH | 6.0 - 9.5 |
| Alcohol dehydrogenase | ADH-160 | GDH, FDH, isopropanol | 6.0 - 9.5 |
| Alcohol dehydrogenase | ADH-171 | GHD | 6.0 - 9.0 |
| Alcohol dehydrogenase | ADH-220 | GDH, FDH, isopropanol | 6.0 - 9.0 |
| Alcohol dehydrogenase | ADH-230 | GDH, FDH, isopropanol | 6.0 - 9.0 |
| Alcohol dehydrogenase | ADH-244 | GDH, isopropanol | 6.0 - 9.0 |
| Glucose dehydrogenase | GDH-5 | - | 6.5 - 9.0 |
| Glucose dehydrognease | GDH-8 | - | 9.0 - 12.0 |
| Glucose dehydrogenase | GDH-101 | - | 6.0 - 9.0 |
| Formate dehydrogenase | FDH-102 | - | 6.0 - 9.0 |
ADHs need a cofactor (NADH or NADPH) for reducing the carbonyl. To be able to use this cofactor in catalytic amounts, the oxidised cofactor has to be continuously reduced, this is called cofactor regeneration. The enzymes accept either or both NAD(P)H cofactors and their regeneration can be achieved by adding a cofactor regeneration enzyme (GDH or FDH, also provided in the kit) and its substrate (D-glucose or sodium formate, respectively). In some cases, the same ADH is able to regenerate the cofactor in presence of isopropanol.
We supply each kit with 200mg of each enzyme, except GDH-101, which is 500mg and also comes with a supply of relevant cofactors.
