C=C double bond reduction kit

The enzymes in this kit have been selected for their activity on a wide selection of substrates and in a wide range of pH, to encounter the different synthetic needs of our customers.

This kit contains 7 enzymes for the reduction of C=C double bonds in presence of an electron withdrawing group (EWG). Examples of electron withdrawing groups are carbonyls (ketones and aldehydes) and nitro. In some specific instances, carboxylic acids, esters and nitriles can also act as activating groups.
The ENEs in this kit include prokaryotic and eukaryotic wild-type enzymes. Most ENEs contain a flavin cofactor (FMN or FAD) that is incorporated in the enzyme at the expression stage and is fundamental for catalysis. Recently a class of ENEs have been characterised that do not require flavin cofactor. The enzyme ENE-105 included in this kit is a member of this class.

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C=C double bond reduction mechanism
Enzyme class  Enzyme Cofactor regeneration Optimum pH
Ene reductase ENE-101  GDH, FDH 6.5 - 8.0 
Ene reductase ENE-102 GDH, FDH 7.0 - 7.5 
Ene reductase ENE-103  GDH, FDH 7.0 - 7.5
Ene reductase ENE-105 GDH 6.0 - 9.0 
Ene reductase ENE-107 GDH, FDH 6.0 - 9.5
Ene reductase ENE-108 GDH, FDH 6.5 - 9.0 
Ene reductase ENE-109 GDH, FDH 6.0 - 8.5 
Glucose dehydrogenase GDH-5         - 6.5 - 9.0           
Glucose dehydrogenase GDH-8         - 9.0 - 12.0
Glucose dehydrogenase GDH-101         - 6.0 - 9.0 
Formate dehydrogenase FDH-102         -  6.0 - 9.0

 

*ENE-105 is also capable to convert substrates with an ester as the electron withdrawing group

 

All ENEs require an additional cofactor (NADH or NADPH) for reducing the C=C double bond. To be able to use this cofactor in catalytic amounts, the oxidised cofactor has to be continuously reduced, this is called cofactor regeneration. The enzymes accept either or both NAD(P)H cofactors and their regeneration can be achieved by adding a cofactor regeneration enzyme (GDH or FDH, also provided in the kit) and its substrate (D-glucose or sodium formate, respectively).

Full enzyme descriptions, reaction setup and work-up instructions, as well as our expert tips, are included with each kit. Please contact us with any questions regarding this kit or its contents and we'll be happy to help.

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Kevin Gwilliam

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    Connie Schlegel

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