Glucose dehydrogenase enzyme technology

Glucose dehydrogenase (GDH enzymes) catalyses the oxidation of D-glucose to D-glucolactone, while reducing in turn NAD+ or NADP+ to NADH and NADPH. The product of this reaction, D-glucolactone, spontaneously and irreversibly hydrolyses in water to gluconic acid, which favors the formation of reduced NADH and NADPH.

Advantages of using glucose dehydrogenase:

GDH enzyme is an efficient system for cofactor regeneration owing to its cheap substrate, the favourable reaction equilibrium towards the reduced cofactor and the high activity of the enzyme.
Easily scalable technology due to the GDH enzyme stability in a wide range of reaction conditions.