Transaminase catalysed reactions generate pyruvate as by-product when alanine is used as amino donor. Even traces of pyruvate in the reaction can cause the reaction equilibrium to stall, therefore it should be removed. This can be achieved with equilibrium-shift enzymes.
We offer two multi-enzymatic systems to remove the pyruvate from the reaction: lactate dehydrogenase (LDH) and alanine dehydrogenase (AlaDH). Both enzymes oxidise in turn NADH to NAD+, which is then regenerated using glucose dehydrogenase (GDH).
